Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control.
Immunogen
TUBA1 antibody was raised against amino acids 427-441 of TUBA1 (Human).
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIFIHCWB
TUBA1 antibody can be used in Western Blot starting at 1 - 2 μg/mL, immunohistochemistry starting at 5 μg/mL, and immunofluorescence.: