Application
Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 μg/mL.Application
Immunofluorescence of Ambra1 in Human Brain cells with Ambra1 antibody at 20 μg/mL.Application
Immunohistochemistry of Ambra1 in human brain with Ambra1 antibody at 5 μg/mL.| Product Name | Ambra1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ambra1 Antibody: DCAF3, WDR94, KIAA1736, Activating molecule in BECN1-regulated autophagy protein 1 |
| Product description | Ambra1 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. Beclin-1, a principal regulator of autophagosome formation, is in turn regulated by Ambra1. Ambra1 associates with Beclin-1 through a region near its center as determined by yeast two-hybrid assay. Null mutations in this gene in mice resulted in embryonic lethality with severe neural tube defects associated with autophagy impairment, accumulation of ubiquitinated proteins, unbalanced cell proliferation and excessive apoptotic death. Furthermore, down-regulation of Ambra1 in cultured cells though RNA interference decreased the level of rapamycin- and nutrient starvation-induced autophagy. Multiple isoforms of Ambra1 are known to exist.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | Ambra1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human Ambra1. The immunogen is located within the last 50 amino acids of Ambra1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AMBRA1 |
|---|---|
| Alternative Names | Ambra1 Antibody: DCAF3 WDR94 KIAA1736 Activating molecule in BECN1-regulated autophagy protein 1 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of Ambra1 in 3T3 cell lysate with Ambra1 antibody at 1 μg/mL.
Application
Immunofluorescence of Ambra1 in Human Brain cells with Ambra1 antibody at 20 μg/mL.
Application
Immunohistochemistry of Ambra1 in human brain with Ambra1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | Ambra1 antibody can be used for the detection of Ambra1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Ambra1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ambra1 Antibody is supplied in PBS containing 0.02% sodium azide. |
