Rabbit polyclonal antibody to Protein LProtein L has the unique ability to bind through kappa light chain interactions without interfering with the antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. Protein L can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. BioVision’s Recombinant Protein L contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. Protein L binds to all classes of Ig (IgG, IgM, IgA, IgE, and IgD). Protein L will also bind Single Chain Variable Fragments (ScFv) and Fab Fragments. Protein L binds kappa I, III, and IV in human and kappa I on mouse.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant Protein L can be used as a positive control.:
Species Reactivity
Other
Concentration
0.5mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
ProteinL Protein L
Target
Protein L
Tissue Specificity
This antibody is specific for Protein L.
Application
Application
Western blot analysis using recombinant Protein L Lane 1: 250 ng Lane 2:1 g
Application Notes
Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Recombinant Protein L can be used as a positive control.:
Additional Information
Form
Liquid
Storage Instructions
The antibody solution should be gently mixed before use. Stored at -20 °C for future use.