Application
Immunohistochemistry of AMIGO1 in human brain tissue with AMIGO1 antibody at 5 μg/mL.Application
Immunofluorescence of AMIGO1 in human brain tissue with AMIGO1 antibody at 20 μg/mL.Application
Western blot analysis of AMIGO1 in HeLa cell lysate with AMIGO1 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | AMIGO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMIGO1 Antibody: ALI2, AMIGO, AMIGO-1, ALI2, KIAA1163, Amphoterin-induced protein 1 |
| Product description | The amphoterin-induced gene and ORF (AMIGO1) protein is a brain-enriched, glycosylated transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRRs) and one Ig-like domain. It and the related proteins AMIGO2 and AMIGO3 are thought to be cell adhesion molecules expressed on fiber tracts of neuronal tissues and participate in their formation (1). AMIGO1 has also been suggested to play important roles in dendritic outgrowth during development and could modulate the survival of developing and adult neurons (2).1) Kuja-Panula J, Kiiltomaki M, Yamashiro T, et al. AMIGO, a transmembrane protein implicated in axon tract development, defines a novel protein family with leucine-rich repeats. J. Cell Biol. 2003; 160:963-73. |
| Immunogen | AMIGO1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AMIGO1. The immunogen is located within the last 50 amino acids of AMIGO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AMIGO1 |
|---|---|
| Alternative Names | AMIGO1 Antibody: ALI2 AMIGO AMIGO-1 ALI2 KIAA1163 Amphoterin-induced protein 1 |
| Molecular Weight(MW) | Predicted: 54 kDa Observed: 55 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | AMIGO1 antibody is human, mouse, and rat reactive. AMIGO1 antibody is predicted to not cross-react with AMIGO2 or AMIGO3. |
Application
Immunohistochemistry of AMIGO1 in human brain tissue with AMIGO1 antibody at 5 μg/mL.
Application
Immunofluorescence of AMIGO1 in human brain tissue with AMIGO1 antibody at 20 μg/mL.
Application
Western blot analysis of AMIGO1 in HeLa cell lysate with AMIGO1 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | AMIGO1 antibody can be used for detection of AMIGO1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AMIGO1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | AMIGO1 antibody is supplied in PBS containing 0.02% sodium azide. |
