Application
Western blot analysis of anti-AMPK alpha2 C-term Pab in mouse heart tissue lysate. AMPK alpha2 was detected using purified Pab.| Product Name | AMPK alpha2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2 |
| Product description | AMPK2 alpha2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.1) Minokoshi, Y., et al., Nature 428(6982):569-574 (2004). |
| Immunogen | This AMPK alpha2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-507 amino acids from the C-terminal region of human AMPK alpha2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PRKAA2 |
|---|---|
| Alternative Names | 5'-AMP-activated protein kinase catalytic subunit alpha-2 AMPK subunit alpha-2 Acetyl-CoA carboxylase kinase ACACA kinase Hydroxymethylglutaryl-CoA reductase kinase HMGCR kinase PRKAA2 AMPK AMPK2 |
| Molecular Weight(MW) | 62 kDa |
Application
Western blot analysis of anti-AMPK alpha2 C-term Pab in mouse heart tissue lysate. AMPK alpha2 was detected using purified Pab.| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
