Application
Western Blot at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of AMPK2 Antibody in Hela cell line lysates (35ug/lane)Application
Western blot analysis of AMPK2 using AMPK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2).| Product Name | AMPK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'-AMP-activated protein kinase catalytic subunit alpha-2, AMPK subunit alpha-2, Acetyl-CoA carboxylase kinase, ACACA kinase, Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, PRKAA2, AMPK, AMPK2 |
| Product description | AMPK2 is a catalytic subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. Studies of the mouse counterpart suggest that this catalytic subunit may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.1) Wyatt,C.N., J. Biol. Chem. 282 (11), 8092-8098 (2007) |
| Immunogen | This AMPK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human AMPK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PRKAA2 |
|---|---|
| Alternative Names | 5'-AMP-activated protein kinase catalytic subunit alpha-2 AMPK subunit alpha-2 Acetyl-CoA carboxylase kinase ACACA kinase Hydroxymethylglutaryl-CoA reductase kinase HMGCR kinase PRKAA2 AMPK AMPK2 |
| Molecular Weight(MW) | 62 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:1000 dilution + 293 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of AMPK2 Antibody in Hela cell line lysates (35ug/lane)
Application
Western blot analysis of AMPK2 using AMPK2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKAA2 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
