Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-174/172). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1/AMPKα2(Ab-174/172).Application
Western blot analysis of lysed extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-174/172).| Product Name | AMPKa1 / AMPKa2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMPK, AMPKa1, MAPTL, MTBT1, TAU, |
| Product description | Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.1) Hurley RL, et al. (2005) J Biol Chem. Aug 12; 280 (32): 29060-29066Woods A, et al. (2003) Curr Biol. Nov 11; 13 (22): 2004-2008Nielsen JN, et al. (2003) J Appl Physiol. Feb; 94 (2): 631-641Da Silva Xavier G, et al. (2000) Proc Natl Acad Sci U S A. Apr 11; 97 (8): 4023-4028. |
| Immunogen | AMPKa1/AMPKa2 (Ab-174/172) antibody was raised against a peptide sequence around aa. 172~176/170~174 (L-R-T-S-C) derived from Human AMPKα1/AMPKα2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKAA1 PRKAA2 |
|---|---|
| Alternative Names | AMPK AMPKa1 MAPTL MTBT1 TAU |
| Molecular Weight(MW) | 63 kDa |
| Tissue Specificity | This antibody detects endogenous level of total AMPKα1/AMPKα2 protein. |
| Entrez Gene | 5562 / 5563 |
|---|---|
| Protein Accession | NP_006242.5 / NP_006243.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AMPKα1/AMPKα2 (Ab-174/172). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using AMPKα1/AMPKα2(Ab-174/172).
Application
Western blot analysis of lysed extracts from A549, HepG2 and 3T3 cells using AMPKα1/AMPKα2 (Ab-174/172).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
