Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Product Name | AMT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AMT, GCE, GCST, NKH, GCVT |
| Product description | The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). Glycine encephalopathy (GCE) may be due to a defect in any one of these enzymes.The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.1) Kure, S., (2006) Hum. Mutat. 27 (4), 343-352. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AMT. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AMT antibody can be used for detection of AMT by ELISA at 1:12500. AMT antibody can be used for detection of AMT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AMT |
|---|---|
| Alternative Names | AMT GCE GCST NKH GCVT |
| Molecular Weight(MW) | 44 kDa |
Application
Antibody used in WB on Human Lung at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate |
|---|---|
| Application Notes | AMT antibody can be used for detection of AMT by ELISA at 1:12500. AMT antibody can be used for detection of AMT by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AMT antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
