Application
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody diluted at 1:500.Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody diluted at 1:500.Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody diluted at 1:1000.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:1000| Product Name | Prothrombin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Prothrombin (coagulation factor II (thrombin))Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Mutations in F2 leads to various forms of thrombosis and dysprothrombinemia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Prothrombin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (96%), Bovine (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PT antibody F2 antibody prothrombin antibody serine protease antibody prothrombin B-chain antibody coagulation factor II (thrombin) antibody |
|---|---|
| Molecular Weight(MW) | 70 kDa (note) (2) |
| Cellular Localization | Secreted , extracellular space |
Application
Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody diluted at 1:500.
Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody diluted at 1:500.
Application
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Prothrombin antibody diluted at 1:1000.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , human plasma , mouse plasma , rat plasma |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
