Application
Western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).Application
ANKFY1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ANKFY1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rabankyrin-5, Rank-5, Ankyrin repeat and FYVE domain-containing protein 1, Ankyrin repeats hooked to a zinc finger motif, ANKFY1, ANKHZN, KIAA1255 |
| Product description | ANKFY1 encodes a cytoplasmic protein that contains a coiled-coil structure and a BTB/POZ domain at its N-terminus, ankyrin repeats in the middle portion, and a FYVE-finger motif at its C-terminus. This protein belongs to a subgroup of double zinc finger proteins which may be involved in vesicle or protein transport.1) Bouslam, N., et al. Hum. Genet. 121 (3-4), 413-420 (2007) |
| Immunogen | This ANKFY1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-465 amino acids from the C-terminal region of human ANKFY1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANKFY1 |
|---|---|
| Alternative Names | Rabankyrin-5 Rank-5 Ankyrin repeat and FYVE domain-containing protein 1 Ankyrin repeats hooked to a zinc finger motif ANKFY1 ANKHZN KIAA1255 |
| Molecular Weight(MW) | 128 kDa |
Application
Western blot analysis in MDA-MB435,CEM,MCF-7,Jurkat,Hela cell line lysates (35ug/lane).This demonstrates the ANKFY1 antibody detected the ANKFY1 protein (arrow).
Application
ANKFY1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
