Application
Western blot analysis in A549 cell line lysates (35ug/lane).Application
ANKRD58 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of ANKRD58 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ANKRD58 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ankyrin repeat domain-containing protein SOWAHD, Ankyrin repeat domain-containing protein 58, Protein sosondowah homolog D, SOWAHD, ANKRD58 |
| Product description | 1) Ross, M.T., et al. Nature 434(7031):325-337(2005) |
| Immunogen | This ANKRD58 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-224 amino acids from the C-terminal region of human ANKRD58. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SOWAHD |
|---|---|
| Alternative Names | Ankyrin repeat domain-containing protein SOWAHD Ankyrin repeat domain-containing protein 58 Protein sosondowah homolog D SOWAHD ANKRD58 |
| Molecular Weight(MW) | 34 kDa |
Application
Western blot analysis in A549 cell line lysates (35ug/lane).
Application
ANKRD58 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of ANKRD58 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
