Application
Immunocytochemistry of Anosmin in MCF7 cells with Anosmin antibody at 5 μg/mL.Application
Western blot analysis of Anosmin in MCF7 cell lysate with Anosmin antibody at 1 μg/mL.Application
Immunofluorescence of Anosmin in MCF7 cells with Anosmin antibody at 20 μg/mL.| Product Name | Anosmin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Anosmin Antibody: HH1, HHA, KAL, KMS, ADMLX, WFDC19, KALIG-1, KALIG1, Anosmin-1, Adhesion molecule-like X-linked |
| Product description | Anosmin Antibody: Mutations in Anosmin-1, an extracellular matrix-associated glycosylated protein, have been linked with Kallmann Syndrome (KS), an X-linked genetic disorder characterized by loss of smell caused by abnormal olfactory bulb development and delayed puberty caused by disrupted migration of the gonadotropin-releasing hormone neuron. Anosmin-1 has been shown to directly bind FGFR1 via its N-terminal cysteine-rich domain, whey-acidic protein-like domain, and its first FnIII repeat with the D2 and D3 ectodomains of FGFR1. It is thought that Anosmin-1 can modulate FGFR1 signaling and have opposing effects on the formation and activation of FGF2-FGFR1-heparing complex.1) Franco B, Guioli S, Pragliola A, et al. A gene deleted in Kallmann’s syndrome shares homology with neural cell adhesion and axonal path-finding molecules. Nature 1991; 353:529-36. |
| Immunogen | Anosmin antibody was raised against an 18 amino acid synthetic peptide near the center of human Anosmin. The immunogen is located within amino acids 430 - 480 of Anosmin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Anosmin antibody can be used for detection of Anosmin by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | KAL1 |
|---|---|
| Alternative Names | Anosmin Antibody: HH1 HHA KAL KMS ADMLX WFDC19 KALIG-1 KALIG1 Anosmin-1 Adhesion molecule-like X-linked |
| Sequence Similarities | 2) Cat. No. 17-012 - MCF7 Cell Slide |
Application
Immunocytochemistry of Anosmin in MCF7 cells with Anosmin antibody at 5 μg/mL.
Application
Western blot analysis of Anosmin in MCF7 cell lysate with Anosmin antibody at 1 μg/mL.
Application
Immunofluorescence of Anosmin in MCF7 cells with Anosmin antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | Anosmin antibody can be used for detection of Anosmin by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Anosmin antibody can be stored at 4 ˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Anosmin Antibody is supplied in PBS containing 0.02% sodium azide. |
