Application
Antibody used in WB on mouse fibroblasts at 1:600.Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in IHC on Human Intestine.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | ANXA1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ANXA1, ANX1, LPC1 |
| Product description | ANXA1 encodes a protein that belongs to a family of Ca (2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity.1) Ernst, S., et al., (2004) J. Immunol. 172 (12), 7669-7676. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ANXA1 |
|---|---|
| Alternative Names | ANXA1 ANX1 LPC1 |
| Molecular Weight(MW) | 38 kDa |
Application
Antibody used in WB on mouse fibroblasts at 1:600.
Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in IHC on Human Intestine.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ANXA1 antibody can be used for detection of ANXA1 by ELISA at 1:62500. ANXA1 antibody can be used for detection of ANXA1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Data sheet for OM271186