ANXA2 encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption. ANXA2 has three pseudogenes located on chromosomes 4, 9 and 10, respectively. Multiple alternatively spliced transcript variants encoding different isoforms have been found for ANXA2.1) Katsunuma, T., et al., (2004) Int. Arch. Allergy Immunol. 134 (1), 29-33.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ANXA2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ANXA2 antibody can be used for detection of ANXA2 by ELISA at 1:312500. ANXA2 antibody can be used for detection of ANXA2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse at: 1:250 (Lane 1: 20ug mouse GL261 cell lysate, Lane 2: 20ug mouse astrocyte cell lysate).
Positive Control
1) Cat. No. XBL-10410 - Fetal Lung Tissue Lysate
Application Notes
ANXA2 antibody can be used for detection of ANXA2 by ELISA at 1:312500. ANXA2 antibody can be used for detection of ANXA2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ANXA2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt