Application
Western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).Application
Western blot analysis of anti-ANXA2 Antibody in mouse lung tissue lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 ug per lane.Application
Western blot analysis of ANXA2 using rabbit polyclonal ANXA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.Application
Antibody staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Product Name | ANXA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Annexin A2, Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANXA2, ANX2, ANX2L4, CAL1H, LPC2D |
| Product description | ANXA2 is a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.1) He,K.L., J. Biol. Chem. 283 (28), 19192-19200 (2008) Tamma,G., Pflugers Arch. 456 (4), 729-736 (2008) Gou,D., J. Biol. Chem. 283 (19), 13156-13164 (2008) |
| Immunogen | This ANXA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-49 amino acids from the N-terminal region of human ANXA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANXA2 |
|---|---|
| Alternative Names | Annexin A2 Annexin II Annexin-2 Calpactin I heavy chain Calpactin-1 heavy chain Chromobindin-8 Lipocortin II Placental anticoagulant protein IV PAP-IV Protein I p36 ANXA2 ANX2 ANX2L4 CAL1H LPC2D |
| Molecular Weight(MW) | 39 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in 293,MDA-MB231 cell line lysates (35ug/lane).
Application
Western blot analysis of anti-ANXA2 Antibody in mouse lung tissue lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ANXA2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: NIH/3T3 whole cell lysates Lane 4: SH-SY5Y whole cell lysates Lane 5: SK-BR-3 whole cell lysates Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of ANXA2 using rabbit polyclonal ANXA2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ANXA2 gene.
Application
Antibody staining ANXA2 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).| Application Notes | For IHC-P starting dilution is: 1:25 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
