Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PRP19 antibody at 1:500 dilution.Application
Sample (30μg whole cell lysate) A:H1299 B:MOLT4 7.5% SDS PAGE diluted at 1:3000Application
PRP19 antibody [C1C3] detects PRP19 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRP19 protein stained by PRP19 antibody [C1C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Product Name | PRP19 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRP19 (PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae))PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRP19. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineMouse (100%), Rat (100%), Xenopus laevis (83%), Pig (99%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NMP200 antibody PRP19 antibody PSO4 antibody SNEV antibody UBOX4 antibody hPSO4 antibody PRPF19 antibody nuclear matrix protein 200 antibody nuclear matrix protein NMP200 related to splicing factor PRP19 antibody senescence evasion factor antibody |
|---|---|
| Molecular Weight(MW) | 55 kDa (note) |
| Cellular Localization | Nucleus , nucleoplasm |
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using PRP19 antibody at 1:500 dilution.
Application
Sample (30μg whole cell lysate) A:H1299 B:MOLT4 7.5% SDS PAGE diluted at 1:3000
Application
PRP19 antibody [C1C3] detects PRP19 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PRP19 protein stained by PRP19 antibody [C1C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
