Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.| Product Name | AP1G1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP1G1, ADTG, CLAPG1, MGC18255 |
| Product description | Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. AP1G1 is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family.Adaptins are important components of clathrin-coated vesicles transporting ligand-receptor complexes from the plasma membrane or from the trans-Golgi network to lysosomes. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. The protein encoded by this gene is a gamma-adaptin protein and it belongs to the adaptor complexes large subunits family. Two transcript variants encoding different isoforms have been found for this gene.1) Rost, M., (2006) J. Biol. Chem. 281 (39), 29297-29308. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human AP1G1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| AP1G1 antibody can be used for detection of AP1G1 by ELISA at 1:312500. AP1G1 antibody can be used for detection of AP1G1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AP1G1 |
|---|---|
| Alternative Names | AP1G1 ADTG CLAPG1 MGC18255 |
| Molecular Weight(MW) | 91 kDa |
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | AP1G1 antibody can be used for detection of AP1G1 by ELISA at 1:312500. AP1G1 antibody can be used for detection of AP1G1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store AP1G1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
