Application
Confocal immunofluorescent analysis of AP1M1 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
AP1M1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody at 1:1000 at each lane.| Product Name | AP1M1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coa |
| Product description | The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. [provided by RefSeq].1) Sawasdee, N., et al. Biochem. Biophys. Res. Commun. 401(1):85-91(2010) |
| Immunogen | This AP1M1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-227 amino acids from the Central region of human AP1M1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AP1M1 |
|---|---|
| Alternative Names | AP-1 complex subunit mu-1 AP-mu chain family member mu1A Adaptor protein complex AP-1 subunit mu-1 Adaptor-related protein complex 1 subunit mu-1 Clathrin assembly protein complex 1 mu-1 medium chain 1 Clathrin coat assembly protein AP47 Clathrin coa |
| Molecular Weight(MW) | 49 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Confocal immunofluorescent analysis of AP1M1 Antibody with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
AP1M1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysates from A375 cell line and rat testis tissue lysate(from left to right), using AP1M1 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
