Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
AP2A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | AP2A1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP-2 complex subunit alpha-1, 100 kDa coated vesicle protein A, Adaptor protein complex AP-2 subunit alpha-1, Adaptor-related protein complex 2 subunit alpha-1, Alpha-adaptin A, Alpha1-adaptin, Clathrin assembly protein complex 2 alpha-A large chain, Plasm |
| Product description | This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined.1) Mitchell, R.S., et al. J. Virol. 82(16):7758-7767(2008) |
| Immunogen | This AP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-735 amino acids from the C-terminal region of human AP2A1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AP2A1 |
|---|---|
| Alternative Names | AP-2 complex subunit alpha-1 100 kDa coated vesicle protein A Adaptor protein complex AP-2 subunit alpha-1 Adaptor-related protein complex 2 subunit alpha-1 Alpha-adaptin A Alpha1-adaptin Clathrin assembly protein complex 2 alpha-A large chain Plasm |
| Molecular Weight(MW) | 108 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
AP2A1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
