ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.WB
Western blot analysis using AP2A1 antibody against Cos-7(1),K562(2),Hepg2(3),THP-1(4),A549(5) cell,Rat spleen tissue(6) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Product Name | Anti-AP2A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Polypeptide |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AP2A1 |
|---|---|
| Gene Synonyms | ADTAA AP2-ALPHA CLAPA1 |
| Gene Full Name | adaptor related protein complex 2 subunit alpha 1 |
| Gene Summary | This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008] |
| Alternative Names | 100 kDa coated vesicle protein A adapter-related protein complex 2 alpha-1 subunit adapter-related protein complex 2 subunit alpha-1 adaptin alpha A adaptor protein complex AP-2 subunit alpha-1 adaptor protein complex AP-2 alpha 1 subunit adaptor related protein complex 2 alpha 1 subunit adaptor-related protein complex 2 subunit alpha-1 adaptor-related protein complex 2 alpha 1 subunit adaptor-related protein complex AP-2 alpha 1 subunit ADTAB alpha-adaptin A alpha-adaptin C alpha1-adaptin alpha2-adaptin AP2 CLAPA2 clathrin assembly protein complex 2 alpha-A large chain clathrin-associated/assembly/adaptor protein large alpha 1 HIP-9 HIP9 HYPJ plasma membrane adaptor HA2/AP2 adaptin alpha A subunit |
| Molecular Weight(MW) | 107kDa |
| Source | Rabbit |
| Cellular Localization | Cell membrane;Membrane, coated pit |
| SwissProt ID | O95782 |
|---|
ELISA
Coat the plate with immunizing antigen.Blue line: Immune serum; Orange line: Pre-immune serum.
WB
Western blot analysis using AP2A1 antibody against Cos-7(1),K562(2),Hepg2(3),THP-1(4),A549(5) cell,Rat spleen tissue(6) . 12% SDS-PAGE gel.Sample loading: 20μg /lane. Transfer the proteins onto a PVDF membrane (OM790003), and block it with TBST (OM750016) plus skimmed milk powder(OM750084) for one hour. Dilute the primary antibody with the antibody diluent (OM750012) at a ratio of 1:1000, and incubate it overnight at 4°C. Wash the membrane three times with TBST (OM750016), 5 minutes each time. At room temperature, dilute the secondary antibody, Goat Anti-Rabbit IgG(H&L)-HRP (OM643487), at a ratio of 1:20000 and incubate for one hour. Wash the membrane three times with TBST (OM750016) again, 5 minutes each time. Use ECL (OM625701) for luminescence.staining time: 60S.| Application Notes | WB:1:1000-1:2000 ELISA:1:1000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. Avoid freeze / thaw cycle. |
| Storage Buffer | Purified antibody in PBS with 0.05% PC300,40% Glycerol. |
