Application
Immunohistochemistry of AP3B2 in mouse brain tissue with AP3B2 antibody at 5 μg/mL.Application
Western blot analysis of AP3B2 in rat brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of AP3B2 in mouse brain tissue with AP3B2 antibody at 20 μg/mL.| Product Name | AP3B2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP3B2 Antibody: NAPTB, AP-3 complex subunit beta-2, Adapter-related protein complex 3 subunit beta-2 |
| Product description | AP3B2 Antibody: The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Unlike the homologous AP3B1, AP3B2-containing AP3 complexes are preferentially targeted to neuronal processes, and mice deficient in AP3B2 displayed compromised synaptic zinc stores. |
| Immunogen | AP3B2 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AP3B2. The immunogen is located within the first 50 amino acids of AP3B2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AP3B2 antibody can be used for detection of AP3B2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AP3B2 |
|---|---|
| Alternative Names | AP3B2 Antibody: NAPTB AP-3 complex subunit beta-2 Adapter-related protein complex 3 subunit beta-2 |
| Molecular Weight(MW) | Predicted: 121 kDa Observed: 128 kDa |
| Entrez Gene | 8120 |
|---|---|
| Protein Accession | NP_001265440 |
Application
Immunohistochemistry of AP3B2 in mouse brain tissue with AP3B2 antibody at 5 μg/mL.
Application
Western blot analysis of AP3B2 in rat brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of AP3B2 in mouse brain tissue with AP3B2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | AP3B2 antibody can be used for detection of AP3B2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AP3B2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AP3B2 Antibody is supplied in PBS containing 0.02% sodium azide. |
