Application
Immunofluorescence of AP3M1 in mouse brain tissue with AP3M1 antibody at 20 μg/mL.Application
Immunohistochemistry of AP3M1 in mouse brain tissue with AP3M1 antibody at 5 μg/mL.Application
Western blot analysis of AP3M1 in human brain tissue lysate with AP3M1 antibody at (A) 1 and (B) 2 μg/mL .| Product Name | AP3M1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP3M1 Antibody: AP-3 complex subunit mu-1, AP-3 adapter complex mu3A subunit |
| Product description | AP3M1 Antibody: The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3M1 is highly homologous to AP3M2 but is the preferred binding partner to the YQRL motif from the cytosolic domain of TGN38. AP3M1 has also been shown to interact with the HIV-1 virulence protein Nef.1) Dell'Angelica EC, Ohno H, Ooi CE, et al. AP-3: an adaptor-like protein complex with ubiquitous expression. EMBO J. 1997; 16:917-28 |
| Immunogen | AP3M1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human AP3M1. The immunogen is located within amino acids 340 - 390 of AP3M1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AP3M1 antibody can be used for detection of AP3M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AP3M1 |
|---|---|
| Alternative Names | AP3M1 Antibody: AP-3 complex subunit mu-1 AP-3 adapter complex mu3A subunit |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
| Tissue Specificity | AP3M1 antibody may cross-react with AP3M2. |
Application
Immunofluorescence of AP3M1 in mouse brain tissue with AP3M1 antibody at 20 μg/mL.
Application
Immunohistochemistry of AP3M1 in mouse brain tissue with AP3M1 antibody at 5 μg/mL.
Application
Western blot analysis of AP3M1 in human brain tissue lysate with AP3M1 antibody at (A) 1 and (B) 2 μg/mL .| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | AP3M1 antibody can be used for detection of AP3M1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AP3M1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AP3M1 Antibody is supplied in PBS containing 0.02% sodium azide. |
