Application
Western blot analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 μg/mL .Application
Immunofluorescence of AP3S1 in human kidney tissue with AP3S1 antibody at 20 μg/mL.Application
Immunohistochemistry of AP3S1 in human kidney tissue with AP3S1 antibody at 2.5 μg/mL.| Product Name | AP3S1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AP3S1 Antibody: CLAPS3, Sigma3A, CLAPS3, AP-3 complex subunit sigma-1, AP-3 complex subunit sigma-3A, Sigma3A-adaptin |
| Product description | AP3S1 Antibody: AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.1) Watanabe TK, Shimizu F, Nagata M, et al. Cloning, expression pattern and mapping to 12p 13.2 --> p13.1 of CLAPS3, a gene encoding a novel clathrin-adaptor small chain. Cytogenet. Cell Genet. 1996; 73:214-7 |
| Immunogen | AP3S1 antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1. The immunogen is located within the first 50 amino acids of AP3S1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| AP3S1 antibody can be used for detection of AP3S1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AP3S1 |
|---|---|
| Alternative Names | AP3S1 Antibody: CLAPS3 Sigma3A CLAPS3 AP-3 complex subunit sigma-1 AP-3 complex subunit sigma-3A Sigma3A-adaptin |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | At least three isoforms of AP3S1 are known to exist; this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members. |
Application
Western blot analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 μg/mL .
Application
Immunofluorescence of AP3S1 in human kidney tissue with AP3S1 antibody at 20 μg/mL.
Application
Immunohistochemistry of AP3S1 in human kidney tissue with AP3S1 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | AP3S1 antibody can be used for detection of AP3S1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AP3S1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AP3S1 Antibody is supplied in PBS containing 0.02% sodium azide. |
