Application
Western blot analysis of APC11 in HeLa cell lysate with APC11 antibody at 1 μg/mL.Application
Immunofluorescence of APC11 in 3T3 cells with APC11 antibody at 20 μg/mL.| Product Name | APC11 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC11 Antibody: APC11, Apc11p, HSPC214, Anaphase-promoting complex subunit 11, Cyclosome subunit 11, APC11 |
| Product description | APC11 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC11 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC11, together with APC2 and Ubc4, form the minimal APC ubiquitin ligase module.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC11 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC11. The immunogen is located within amino acids 70 - 120 of APC11. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| APC11 antibody can be used for detection of APC11 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANAPC11 |
|---|---|
| Alternative Names | APC11 Antibody: APC11 Apc11p HSPC214 Anaphase-promoting complex subunit 11 Cyclosome subunit 11 APC11 |
| Molecular Weight(MW) | Predicted: 22 kDa Observed: 22 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Entrez Gene | 51529 |
|---|---|
| Protein Accession | NP_001002244 |
Application
Western blot analysis of APC11 in HeLa cell lysate with APC11 antibody at 1 μg/mL.
Application
Immunofluorescence of APC11 in 3T3 cells with APC11 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | APC11 antibody can be used for detection of APC11 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC11 Antibody is supplied in PBS containing 0.02% sodium azide. |
