Application
Immunofluorescence of APC13 in Jurkat cells with APC13 antibody at 20 μg/mL.Application
Western blot analysis of (A) 1 ng and (B) 5 ng of Transthyretin recombinant protein with Transthyretin antibody at 1 μg/mL.Application
Western blot analysis of APC13 in Jurkat cell tissue lysate with APC13 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | APC13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC13 Antibody: SWM1, APC13, Anaphase-promoting complex subunit 13, Cyclosome subunit 13 |
| Product description | APC13 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC13 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. APC13 promotes the stable association of APC3/Cdc27 and APC6/Cdc16 with the APC/C.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC13 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC13. The immunogen is located within the last 50 amino acids of APC13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| APC13 antibody can be used for detection of APC13 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANAPC13 |
|---|---|
| Alternative Names | APC13 Antibody: SWM1 APC13 Anaphase-promoting complex subunit 13 Cyclosome subunit 13 |
| Molecular Weight(MW) | Predicted: 15 kDa Observed: 14 kDa |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunofluorescence of APC13 in Jurkat cells with APC13 antibody at 20 μg/mL.
Application
Western blot analysis of (A) 1 ng and (B) 5 ng of Transthyretin recombinant protein with Transthyretin antibody at 1 μg/mL.
Application
Western blot analysis of APC13 in Jurkat cell tissue lysate with APC13 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | APC13 antibody can be used for detection of APC13 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC13 Antibody is supplied in PBS containing 0.02% sodium azide. |
