Application
Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.Application
Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.| Product Name | APC2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC2 Antibody: APCL, APCL, Adenomatous polyposis coli protein 2, Adenomatous polyposis coli protein-like, APC-like |
| Product description | APC2 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC2 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. Like APC11, APC2 contains cullin and RING finger domains that are thought to be important in regulating ubiquitination activity.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC2 antibody was raised against an 18 amino acid synthetic peptide near the center of human APC2. The immunogen is located within amino acids 240 - 290 of APC2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APC2 |
|---|---|
| Alternative Names | APC2 Antibody: APCL APCL Adenomatous polyposis coli protein 2 Adenomatous polyposis coli protein-like APC-like |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Western blot analysis of APC2 in HeLa cell lysate with APC2 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of APC2 in HeLa cells with APC2 antibody at 20 μg/mL.
Application
Immunocytochemistry of APC2 in HeLa cells with APC2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | APC2 antibody can be used for detection of APC2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC2 Antibody is supplied in PBS containing 0.02% sodium azide. |
