Application
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunohistochemistry of APC3 in rat liver tissue with APC3 antibody at 5 μg/mL.| Product Name | APC3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC3 Antibody: APC3, HNUC, NUC2, ANAPC3, CDC27Hs, D0S1430E, D17S978E, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3 |
| Product description | APC3 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC3, also known as CDC27, a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), is a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. APC3 contains a tetratricopeptide repeat (TPR) region and interacts with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC3 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human APC3. The immunogen is located within the last 50 amino acids of APC3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| APC3 antibody can be used for detection of APC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDC27 |
|---|---|
| Alternative Names | APC3 Antibody: APC3 HNUC NUC2 ANAPC3 CDC27Hs D0S1430E D17S978E Cell division cycle protein 27 homolog Anaphase-promoting complex subunit 3 APC3 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Western blot analysis of APC3 in mouse liver tissue lysate with APC3 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunohistochemistry of APC3 in rat liver tissue with APC3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1404 - Mouse Liver Tissue Lysate |
|---|---|
| Application Notes | APC3 antibody can be used for detection of APC3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC3 Antibody is supplied in PBS containing 0.02% sodium azide. |
