Application
Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.Application
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.Application
Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | APC5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APC5 Antibody: APC5, APC5, Anaphase-promoting complex subunit 5, Cyclosome subunit 5 |
| Product description | APC5 Antibody: Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.1) JM Peters. The anaphase promoting complex/cyclosome: a machine designed to destroy. Nat. Rev. Mol. Cell Biol.2006; 7:644-56. |
| Immunogen | APC5 antibody was raised against a 17 amino acid synthetic peptide near the center of human APC5. The immunogen is located within amino acids 480 - 530 of APC5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-P |
| APC5 antibody can be used for detection of APC5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANAPC5 |
|---|---|
| Alternative Names | APC5 Antibody: APC5 APC5 Anaphase-promoting complex subunit 5 Cyclosome subunit 5 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Chicken: (100%) |
Application
Immunofluorescence of APC5 in rat kidney tissue with APC5 antibody at 20 μg/mL.
Application
Immunohistochemistry of APC5 in rat kidney tissue with APC5 antibody at 5 μg/mL.
Application
Western blot analysis of APC5 in human kidney tissue lysate with APC5 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | APC5 antibody can be used for detection of APC5 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APC5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APC5 Antibody is supplied in PBS containing 0.02% sodium azide. |
