Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.| Product Name | APEH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APEH, ACPH, APH, D3F15S2, D3S48E, DNF15S2, MGC2178, OPH, AARE |
| Product description | APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma.This gene encodes the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. This gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. It can play an important role in destroying oxidatively damaged proteins in living cells. Deletions of this gene locus are found in various types of carcinomas, including small cell lung carcinoma and renal cell carcinoma. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Pope, S.N. J. Steroid Biochem. Mol. Biol. 94 (1-3), 203-208 (2005). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APEH. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| APEH antibody can be used for detection of APEH by ELISA at 1:312500. APEH antibody can be used for detection of APEH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APEH |
|---|---|
| Alternative Names | APEH ACPH APH D3F15S2 D3S48E DNF15S2 MGC2178 OPH AARE |
| Molecular Weight(MW) | 81 kDa |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | APEH antibody can be used for detection of APEH by ELISA at 1:312500. APEH antibody can be used for detection of APEH by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store APEH antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
