Application
Western blot analysis of APEH antibody in A2058 cell line lysates (35ug/lane)| Product Name | APEH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acylamino-acid-releasing enzyme, AARE, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase, Oxidized protein hydrolase, OPH, APEH, D3F15S2, D3S48E, DNF15S2 |
| Product description | APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. APEH gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. The protein can play an important role in destroying oxidatively damaged proteins in living cells.1) Perrier,J., Giardina,T. Biol. Cell 94 (1), 45-54 (2002) |
| Immunogen | This APEH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-204 amino acids from the N-terminal region of human APEH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | APEH |
|---|---|
| Alternative Names | Acylamino-acid-releasing enzyme AARE Acyl-peptide hydrolase APH Acylaminoacyl-peptidase Oxidized protein hydrolase OPH APEH D3F15S2 D3S48E DNF15S2 |
| Molecular Weight(MW) | 81 kDa |
Application
Western blot analysis of APEH antibody in A2058 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
