Application
Confocal immunofluorescent analysis of APEX1 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of anti-APEX1 Antibody in Hela cell line lysates (35ug/lane)Application
Western blot analysis of APEX1 using rabbit polyclonal APEX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.| Product Name | APEX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA-(apurinic or apyrimidinic site) lyase, 31--, APEX nuclease, APEN, Apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial, APEX1, APE, APE1, APEX, APX, HAP1, REF1 |
| Product description | Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.1) Vascotto,C., Mol. Cell. Biol. 29 (7), 1834-1854 (2009) |
| Immunogen | This APEX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-64 amino acids from the N-terminal region of human APEX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | APEX1 |
|---|---|
| Alternative Names | DNA-(apurinic or apyrimidinic site) lyase 31-- APEX nuclease APEN Apurinic-apyrimidinic endonuclease 1 AP endonuclease 1 APE-1 REF-1 Redox factor-1 DNA-(apurinic or apyrimidinic site) lyase mitochondrial APEX1 APE APE1 APEX APX HAP1 REF1 |
| Molecular Weight(MW) | 36 kDa |
Application
Confocal immunofluorescent analysis of APEX1 Antibody with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of anti-APEX1 Antibody in Hela cell line lysates (35ug/lane)
Application
Western blot analysis of APEX1 using rabbit polyclonal APEX1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.| Application Notes | For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
