Application
Immunohistochemistry of APH1 in human brain tissue with APH1 antibody at 5 μg/mL.Application
Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | APH1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APH1 Antibody: APH-1, APH-1A, CGI-78, 6530402N02Rik, PSF, UNQ579/PRO1141, Gamma-secretase subunit APH-1A, Aph-1alpha, APH-1a |
| Product description | APH1 Antibody: APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.1) Goutte C, Tsunozaki M, Hale VA, et al. APH-1 is a multipass membrane protein essential for the Notch signaling pathway in Caenorhabditis elegans embryos. Proc. Natl. Acad. Sci. USA2002; 99:775-9. |
| Immunogen | APH1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human APH1. The immunogen is located within amino acids 80 - 130 of APH1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APH1A |
|---|---|
| Alternative Names | APH1 Antibody: APH-1 APH-1A CGI-78 6530402N02Rik PSF UNQ579/PRO1141 Gamma-secretase subunit APH-1A Aph-1alpha APH-1a |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 28 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of APH1 in human brain tissue with APH1 antibody at 5 μg/mL.
Application
Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APH1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | APH1 Antibody is supplied in PBS containing 0.02% sodium azide. |
