Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Product Name | API5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Apoptosis inhibitor 5, API-5, Antiapoptosis clone 11 protein, AAC-11, Cell migration-inducing gene 8 protein, Fibroblast growth factor 2-interacting factor, FIF, Protein XAGL, API5 |
| Product description | Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs.1) Wang, Z., et al. Med. Sci. Monit. 16 (8), CR357-CR364 (2010) : |
| Immunogen | This API5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-510 amino acids from the C-terminal region of human API5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | API5 |
|---|---|
| Alternative Names | Apoptosis inhibitor 5 API-5 Antiapoptosis clone 11 protein AAC-11 Cell migration-inducing gene 8 protein Fibroblast growth factor 2-interacting factor FIF Protein XAGL API5 |
| Molecular Weight(MW) | 59 kDa |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
