Application
Western blot analysis of APITD1 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
APITD1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | APITD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Centromere protein S, CENP-S, Apoptosis-inducing TAF9-like domain-containing protein 1, FANCM-interacting histone fold protein 1, Fanconi anemia-associated polypeptide of 16 kDa, APITD1, CENPS, FAAP16, MHF1 |
| Product description | APITD1 was identified in the neuroblastoma tumour suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumours, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway.1) Amano, M., et al. J. Cell Biol. 186(2):173-182(2009) |
| Immunogen | This APITD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 48-74 amino acids from the Central region of human APITD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APITD1 |
|---|---|
| Alternative Names | Centromere protein S CENP-S Apoptosis-inducing TAF9-like domain-containing protein 1 FANCM-interacting histone fold protein 1 Fanconi anemia-associated polypeptide of 16 kDa APITD1 CENPS FAAP16 MHF1 |
| Molecular Weight(MW) | 16 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of APITD1 Antibody in mouse NIH-3T3 cell line lysates (35ug/lane)
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
APITD1 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
