Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of APOBEC3F Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with APOBEC3F Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | APOBEC3F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | DNA dC->dU-editing enzyme APOBEC-3F, 354-, Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F, A3F, APOBEC3F |
| Product description | This protein is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control.1) Khatua,A.K., et.al., Virology 400 (1), 68-75 (2010) |
| Immunogen | This APOBEC3F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-48 amino acids from the N-terminal region of human APOBEC3F. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APOBEC3F |
|---|---|
| Alternative Names | DNA dC->dU-editing enzyme APOBEC-3F 354- Apolipoprotein B mRNA-editing enzyme catalytic polypeptide-like 3F A3F APOBEC3F |
| Molecular Weight(MW) | 45 kDa |
Application
Flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of APOBEC3F Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with APOBEC3F Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
