APOBEC3G is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. It is thought that the proteins may be RNA editing enzymes and have roles in growth or cell cycle control. The protein encoded by this gene has been found to be a specific inhibitor of human immunodeficiency virus-1 (HIV-1) infectivity.1) Rose, K.M., et al., (2004) Biol. Chem. 279 (40), 41744-41749.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human APOBEC3G.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
IHCWB
APOBEC3G antibody can be used for detection of APOBEC3G by ELISA at 1:1562500. APOBEC3G antibody can be used for detection of APOBEC3G by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
APOBEC3G antibody can be used for detection of APOBEC3G by ELISA at 1:1562500. APOBEC3G antibody can be used for detection of APOBEC3G by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store APOBEC3G antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.