Application
Western blot analysis of APPBP2 in human brain tissue lysate with APPBP2 antibody at (A) 0.5 and (B) 1 μg/ml.Application
Immunofluorescence of APPBP2 in human brain tissue with APPBP2 antibody at 20 μg/mL.Application
Immunohistochemistry of APPBP2 in human brain tissue with APPBP2 antibody at 5 μg/mL.| Product Name | APPBP2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | APPBP2 Antibody: PAT1, APP-BP2, HS.84084, KIAA0228, PAT1, Amyloid protein-binding protein 2, Amyloid beta precursor protein-binding protein 2 |
| Product description | The amyloid beta precursor protein (cytoplasmic tail) binding protein 2 (APPBP2), also known as PAT1, interacts with microtubules and is functionally associated with beta-amyloid precursor protein transport and/or processing (1). The beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease (2). APPBP2 has been found to be highly expressed in breast cancer (3).1) Zheng P, Eastman J, Vande Pol S, et al. PAT1, a microtubule-interacting protein, recognizes the basolateral sorting signal of amyloid precursor protein. Proc. Natl. Acad. Sci. USA 1994; 95:14745-50. |
| Immunogen | APPBP2 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human APPBP2. The immunogen is located within the last 50 amino acids of APPBP2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| APPBP2 antibody can be used for detection of APPBP2 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | APPBP2 |
|---|---|
| Alternative Names | APPBP2 Antibody: PAT1 APP-BP2 HS.84084 KIAA0228 PAT1 Amyloid protein-binding protein 2 Amyloid beta precursor protein-binding protein 2 |
| Molecular Weight(MW) | Predicted: 64 kDa Observed: 57 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | APPBP2 antibody is human, mouse and rat reactive. At least two isoforms of APPBP2 are known to exist; this antibody will detect both isoforms. |
Application
Western blot analysis of APPBP2 in human brain tissue lysate with APPBP2 antibody at (A) 0.5 and (B) 1 μg/ml.
Application
Immunofluorescence of APPBP2 in human brain tissue with APPBP2 antibody at 20 μg/mL.
Application
Immunohistochemistry of APPBP2 in human brain tissue with APPBP2 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | APPBP2 antibody can be used for detection of APPBP2 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | APPBP2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | APPBP2 antibody is supplied in PBS containing 0.02% sodium azide. |
