Application
Western blot analysis of ARC in (H) HeLa, (K) K562, and (A) A549 whole cell lysates with ARC antibody at 1 μg/mL.Application
Immunohistochemistry of ARC in human heart tissue with ARC antibody at 5 μg/mL.Application
Immunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 μg/mL.| Product Name | ARC Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARC Antibody: ARC, FCM, MYP, NOP, NOP30, ARC, Nucleolar protein 3, Apoptosis repressor with CARD, Myp |
| Product description | ARC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.1) Koseki T, Inohara N, Chen S, et al. ARC, an inhibitor of apoptosis expressed in skeletal muscle and heart that interacts selectively with caspases. Proc. Natl. Acad. Sci. USA 1998; 95:5156-60. |
| Immunogen | ARC antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ARC. The immunogen is located within the last 50 amino acids of ARC. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ARC antibody can be used for detection of ARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOL3 |
|---|---|
| Alternative Names | ARC Antibody: ARC FCM MYP NOP NOP30 ARC Nucleolar protein 3 Apoptosis repressor with CARD Myp |
| Molecular Weight(MW) | Predicted: 23 kDa Observed: 25 kDa |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Western blot analysis of ARC in (H) HeLa, (K) K562, and (A) A549 whole cell lysates with ARC antibody at 1 μg/mL.
Application
Immunohistochemistry of ARC in human heart tissue with ARC antibody at 5 μg/mL.
Application
Immunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ARC antibody can be used for detection of ARC by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARC antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ARC Antibody is supplied in PBS containing 0.02% sodium azide. |
