Application
Western blot analysis of Nop30 in mouse muscle tissue lysate with Nop30 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | Nop30 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nop30 Antibody: ARC, FCM, MYP, NOP, NOP30, ARC, Nucleolar protein 3, Apoptosis repressor with CARD, Myp |
| Product description | Nop30 Antibody: Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and the caspase family of proteases. CARD domain containing cell death regulators include RAIDD, Apaf-1, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified and designated ARC for apoptosis repressor with CARD. An alternate splicing isoform of ARC was identified as Nop30. While ARC interacts with caspase-2 and -8 and suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1 and DR3, Nop30 multimerizes and binds to the splicing factor SRp30c and may act to influence alternative splice site selection in vivo. The Nop30 antibody will not detect ARC protein.1) Jin Z and El Deiry WS. Overview of cell death signaling pathways. Cancer Biol. Ther. 2004; 4:139-63. |
| Immunogen | Nop30 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nop30. The immunogen is located within the last 50 amino acids of Nop30. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Nop30 antibody can be used for detection of Nop30 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NOL3 |
|---|---|
| Alternative Names | Nop30 Antibody: ARC FCM MYP NOP NOP30 ARC Nucleolar protein 3 Apoptosis repressor with CARD Myp |
| Entrez Gene | 8996 |
|---|---|
| Protein Accession | NP_001171986 |
Application
Western blot analysis of Nop30 in mouse muscle tissue lysate with Nop30 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1407 - Mouse Skeletal Muscle Tissue Lysate |
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| Application Notes | Nop30 antibody can be used for detection of Nop30 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Nop30 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Nop30 Antibody is supplied in PBS containing 0.02% sodium azide. |
