Application
Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 μg/mL.Application
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 μg/mL.Application
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 μg/mL.| Product Name | ARF-BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARF-BP1 Antibody: MULE, Ib772, LASU1, UREB1, HECTH9, URE-B1, ARF-BP1, HSPC272, KIAA0312, KIAA1578, E3 ubiquitin-protein ligase HUWE1, ARF-binding protein 1 |
| Product description | ARF-BP1 Antibody: The ARF tumor suppressor is a critical regulator of p53 stability. In addition to p53, ARF1 binds to other proteins such as MDM2 and ARF-BP1, a large protein containing HECT, UBA and WWE motifs. ARF-BP1 directly binds and ubiquitinates p53; this activity is inhibited by ARF, indicating that ARF-BP1 is a critical mediator of the p53-dependent and p53-independent tumor suppressor functions of ARF. ARF-BP1 can also catalyze the polyubiquitination of Mcl-1, an anti-apoptotic Bcl-2 family member involved in DNA damage-induced apoptosis. Elimination of ARF-BP1 expression by RNA interference stabilized Mcl-1 protein, resulting in an attenuation of apoptosis induced by DNA-damage agents.1) Gallagher SJ, Kefford RF and Rizos H. The ARF tumour suppressor. Int. J. Biochem. Cell Biol.2006; 38:1637-41. |
| Immunogen | ARF-BP1 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ARF-BP1. The immunogen is located within amino acids 4250 - 4300 of ARF-BP1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| WB:1:500~1:1000 ICC/IF:1:50~1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HUWE1 |
|---|---|
| Alternative Names | ARF-BP1 Antibody: MULE Ib772 LASU1 UREB1 HECTH9 URE-B1 ARF-BP1 HSPC272 KIAA0312 KIAA1578 E3 ubiquitin-protein ligase HUWE1 ARF-binding protein 1 |
Application
Western blot analysis of ARF-BP1 in Daudi cell lysate with ARF-BP1 antibody at 1 μg/mL.
Application
Immunofluorescence of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 20 μg/mL.
Application
Immunocytochemistry of ARF-BP1 in Daudi cells with ARF-BP1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | WB:1:500~1:1000 ICC/IF:1:50~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | ARF-BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ARF-BP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
