Application
Western blot analysis of LDLRAP1 Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LDLRAP1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ARH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Low density lipoprotein receptor adapter protein 1, Autosomal recessive hypercholesterolemia protein, LDLRAP1, ARH |
| Product description | LDLRAP1 is a cytosolic protein which contains a phosphotyrosine binding (PTD) domain. The PTD domain has been found to interact with the cytoplasmic tail of the LDL receptor. This adapter protein is required for efficient endocytosis of the LDL receptor (LDLR) in polarized cells such as hepatocytes and lymphocytes, but not in non-polarized cells (fibroblasts). LDLRAP1 may be required for LDL binding and internalization but not for receptor clustering in coated pits. This protein may facilitate the endocytocis of LDLR and LDLR-LDL complexes from coated pits by stabilizing the interaction between the receptor and the structural components of the pits, and may also be involved in the internalization of other LDLR family members. Mutations in the LDLRAP1 gene lead to LDL receptor malfunction and cause the disorder autosomal recessive hypercholesterolaemia.1) Maurer,M.E., J. Cell. Sci. 119 (PT 20), 4235-4246 (2006) |
| Immunogen | This ARH (LDLRAP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-306 amino acids from the C-terminal region of human ARH (LDLRAP1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | LDLRAP1 |
|---|---|
| Alternative Names | Low density lipoprotein receptor adapter protein 1 Autosomal recessive hypercholesterolemia protein LDLRAP1 ARH |
| Molecular Weight(MW) | 34 kDa |
Application
Western blot analysis of LDLRAP1 Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with LDLRAP1 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
