Application
Western blot analysis of ARHGAP18 in 3T3 cell lysate with ARHGAP18 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ARHGAP18 in 3T3 cells with ARHGAP18 antibody at 20 μg/mL.Application
Immunocytochemistry of ARHGAP18 in 3T3 cells with ARHGAP18 antibody at 2.5 μg/mL.| Product Name | ARHGAP18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARHGAP18 Antibody: SENEX, MacGAP, bA307O14.2, Rho GTPase-activating protein 18 |
| Product description | ARHGAP18 Antibody: ARHGAP18 is one member of the human RhoGAP family with approximately 80 RhoGAP proteins known to be encoded in the human genome. Rho proteins belong to the Ras superfamily that is composed of over 50 members divided into six families, including Ras, Sar, Rho, Ran, Rab and Arf. Rho GTPases are important regulators of the actin cytoskeleton and consequently influence the shape and migration of cells. ARHGAP18 is linked to Ras, and thus, to EGFR-mediated proliferation, migration and differentiation. ARHGAP18 is precisely contained within chromosome 6q22-24, which has been shown to be linked to schizophrenia, suggesting that ARHGEP18 may play a role in this condition.1) Takai Y, Sasaki T, and Matozaki T. Small GTP-binding proteins. Physiol. Rev.2001; 81:153-208. |
| Immunogen | ARHGAP18 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ARHGAP18. The immunogen is located within the last 50 amino acids of ARHGAP18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ARHGAP18 antibody can be used for detection of ARHGAP18 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGAP18 |
|---|---|
| Alternative Names | ARHGAP18 Antibody: SENEX MacGAP bA307O14.2 Rho GTPase-activating protein 18 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
Application
Western blot analysis of ARHGAP18 in 3T3 cell lysate with ARHGAP18 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ARHGAP18 in 3T3 cells with ARHGAP18 antibody at 20 μg/mL.
Application
Immunocytochemistry of ARHGAP18 in 3T3 cells with ARHGAP18 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | ARHGAP18 antibody can be used for detection of ARHGAP18 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARHGAP18 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ARHGAP18 Antibody is supplied in PBS containing 0.02% sodium azide. |
