ARHGDIG is highly expressed in the entire brain, with regional variations. The mRNA is also present at high levels in kidney and pancreas and at moderate levels in spinal cord, stomach, and pituitary gland. ARHGDIG is mapped to chromosome band 16p13.3 which is rich in deletion mutants of genes involved in several human diseases, notably polycystic kidney disease, alpha-thalassemia, tuberous sclerosis, mental retardation, and cancer.1) Adra, C.N., et al., (1998) Genomics 53 (1), 104-109.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARHGDIG.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ARHGDIG antibody can be used for detection of ARHGDIG by ELISA at 1:62500. ARHGDIG antibody can be used for detection of ARHGDIG by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Daudi at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1224 - Daudi Cell Lysate
Application Notes
ARHGDIG antibody can be used for detection of ARHGDIG by ELISA at 1:62500. ARHGDIG antibody can be used for detection of ARHGDIG by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ARHGDIG antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.