Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ARHGEF7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rho guanine nucleotide exchange factor 7, Beta-Pix, COOL-1, PAK-interacting exchange factor beta, p85, ARHGEF7, COOL1, KIAA0142, P85SPR, PAK3BP, PIXB |
| Product description | Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.1) Nagase T.,et al.DNA Res. 2:167-174(1995). |
| Immunogen | This ARHGEF7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 654-688 amino acids from human ARHGEF7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARHGEF7 |
|---|---|
| Alternative Names | Rho guanine nucleotide exchange factor 7 Beta-Pix COOL-1 PAK-interacting exchange factor beta p85 ARHGEF7 COOL1 KIAA0142 P85SPR PAK3BP PIXB |
| Molecular Weight(MW) | 90 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
