Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.| Product Name | Arhgef9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Rho guanine nucleotide exchange factor 9, Collybistin, Rac/Cdc42 guanine nucleotide exchange factor 9, Arhgef9 |
| Product description | ARHGEF9 belongs to a family of Rho-like GTPases that act as molecular switches by cycling from the active GTP-bound state to the inactive GDP-bound state. These proteins are key regulators of the actin cytoskeleton and are involved in cell signaling.1) Xiang,S., J. Mol. Biol. 359 (1), 35-46 (2006) |
| Immunogen | This Arhgef9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 274-303 amino acids from the Central region of human Arhgef9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Arhgef9 |
|---|---|
| Alternative Names | Rho guanine nucleotide exchange factor 9 Collybistin Rac/Cdc42 guanine nucleotide exchange factor 9 Arhgef9 |
| Molecular Weight(MW) | 58 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with Arhgef9 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution Lane 1: rat brain lysate Lane 2: rat heart lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
