Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Product Name | ARID4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARID4A, RBP1, RBBP1, RBP-1, RBBP-1 |
| Product description | ARID4A encodes a ubiquitously expressed nuclear protein. It binds directly, with several other proteins, to retinoblastoma protein (pRB) which regulates cell proliferation. pRB represses transcription by recruiting the encoded protein. This protein, in turn, serves as a bridging molecule to recruit HDACs and, in addition, provides a second HDAC-independent repression function. The encoded protein possesses transcriptional repression activity. Multiple alternatively spliced transcripts have been observed for this gene, although not all transcript variants have been fully described.1) Meehan, W.J., et al., (2004) J. Biol. Chem. 279 (2), 1562-1569. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARID4A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ARID4A antibody can be used for detection of ARID4A by ELISA at 1:62500. ARID4A antibody can be used for detection of ARID4A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ARID4A |
|---|---|
| Alternative Names | ARID4A RBP1 RBBP1 RBP-1 RBBP-1 |
| Molecular Weight(MW) | 143 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ARID4A antibody can be used for detection of ARID4A by ELISA at 1:62500. ARID4A antibody can be used for detection of ARID4A by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ARID4A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
