Application
Immunofluorescence analysis of methanol-fixed HeLa, using PRX I antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000Application
PRX I antibody [N1C2] detects PRX I protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PRX I antibody [N1C2] dilution: 1:500.| Product Name | PRX I antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PRX I (peroxiredoxin 1)This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Three transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PRX I. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, Dog, Chicken, Rhesus Monkey, BovineZebrafish (80%), Dog (97%), Chicken (86%), Rhesus Monkey (98%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSP23 antibody NKEFA antibody PAG antibody PAGA antibody PAGB antibody PRX1 antibody PRXI antibody TDPX2 antibody PRDX1 antibody peroxiredoxin-1 antibody NKEF-A antibody natural killer cell-enhancing factor A antibody thioredoxin peroxidase 2 a |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (4) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PRX I antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A:NIH-3T3 12% SDS PAGE diluted at 1:1000
Application
PRX I antibody [N1C2] detects PRX I protein at cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PRX I antibody [N1C2] dilution: 1:500.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
