ARNTL2-CLOCK heterodimers activate E-box element (3'-CACGTG-5') transcription. Also, in umbilical vein endothelial cells, ARNTL2 activates SERPINE1 through E-box sites. This transactivation is inhibited by PER2 and CRY1.1) Shi, J., (er) Am. J. Med. Genet. B Neuropsychiatr. Genet. (2008) In press.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARNTL2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ARNTL2 antibody can be used for detection of ARNTL2 by ELISA at 1:1562500. ARNTL2 antibody can be used for detection of ARNTL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in IHC on Human Placenta at 5 ug/ml.
Positive Control
1) Cat. No. 1219 - MCF7 Cell Lysate
Application Notes
ARNTL2 antibody can be used for detection of ARNTL2 by ELISA at 1:1562500. ARNTL2 antibody can be used for detection of ARNTL2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ARNTL2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.