Application
Western blot analysis in K562 cell line lysates (35ug/lane).Application
Western blot analysis of ARSF using rabbit polyclonal ARSF Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARSF gene.Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ARSF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arylsulfatase F, ASF, 316-, ARSF |
| Product description | This gene is a member of the sulfatase family, and more specifically, the arylsulfatase subfamily. Members of the subfamily share similarity in sequence and splice sites, and are clustered together on chromosome X, suggesting that they are derived from recent gene duplication events. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin.1) Cao, W., et al. J. Biol. Chem. 273(32):20629-20635(1998) |
| Immunogen | This ARSF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 236-264 amino acids from the Central region of human ARSF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARSF |
|---|---|
| Alternative Names | Arylsulfatase F ASF 316- ARSF |
| Molecular Weight(MW) | 66 kDa |
Application
Western blot analysis in K562 cell line lysates (35ug/lane).
Application
Western blot analysis of ARSF using rabbit polyclonal ARSF Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ARSF gene.
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
