Application
To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect hArtemin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Artemin (38-107) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin.| Product Name | Artemin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | EVN, NBN, ENOVIN, EVN, Artemin, Enovin, |
| Product description | Artemin has been shown in culture to support the survival of a number of peripheral neuron populations and at least one population of dopaminergic CNS neurons. Its role in the PNS and CNS is further substantiated by its expression pattern in the proximity of these neurons. |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hArtemin. Human Artemin specific antibody was purified by affinity chromatography employing immobilized hArtemin matrix. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: Indirect: To detect hArtemin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin. Sandwich: To detect hArtemin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot: To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARTN |
|---|---|
| Alternative Names | EVN NBN ENOVIN EVN Artemin Enovin |
Application
To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect hArtemin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human Artemin (38-107) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin.| Application Notes | ELISA: Indirect: To detect hArtemin by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hArtemin. Sandwich: To detect hArtemin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. Western Blot: To detect hArtemin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hArtemin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Artemin antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
